Abstract: Due to the complex structure, various parts and complicated assembly process of marine diesel engine, it will lead to assembly line balancing problem when reorganizing the assembly process and redistributing assembly operations in the process of transforming traditional assembly line into pulsating assembly line. To address this problem, this paper proposes a balanced allocation method of assembly operations in pulsating assembly line stations. With consideration of the factors such as assembly line beat, process priority constraints and the number of teams, the mathematical model of assembly line balancing optimization in the station is established and a set of rules of assembly assignment for assembly line balance problem are formulated. An optimization algorithm based on hybrid genetic algorithm (GA) and ant colony optimization (ACO) is proposed to solve the mathematical model. Finally, the effectiveness of the algorithm is verified by a case study and analysis.