Abstract: Aiming at the problems of limited experimental equipment; According to the digital twin technology, the logistics and transportation equipment was studied and a virtual simulation experiment system was designed through the Unity3D virtual reality development platform. According to the existing logistics and transportation equipment, including conveying materials, stacker storage and other equipment as the research object, the use of SolidWorks and 3DMax to complete the establishment of the three-dimensional model of the experimental equipment, the use of S7-PLCSIM Advancde to complete the control program of the logistics equipment, through the PLC and Unity3D communication and data interaction, and finally in the virtual environment of Unity3D to complete the virtual simulation experimental system of logistics transportation. Through the system, the logistics and transportation process such as material transfer, warehousing and outbound can be realized in the virtual space, and the virtual visualization of the logistics and transportation work process can be realized, and the working action of logistics equipment can be completed through the PLC program written, which can effectively solve the problems of insufficient experimental equipment, so that the trainers can get the due experimental operation and improve the experimental efficiency.