Abstract: In order to improve the accuracy and comprehensiveness of the input load for the precision retention acceleration test of CNC machine tools, a method for compiling the precision retention acceleration test program spectrum for actual cutting conditions is proposed. Firstly, synchronize the collection of multi-source signals such as cutting force, vibration, temperature, feed rate, and coordinates through force gauges, multi-source information monitoring systems, and numerical control systems, and perform signal preprocessing. Secondly, based on this, load spectra under typical operating conditions were constructed using methods such as rainflow counting and power spectral density analysis. Thirdly, combined with the accelerated precision-retention test method, a "four-stress and three-level" accelerated precision retention test program spectrum was established, which includes static and dynamic forces, vibration, temperature, and feed speed. Finally, a complete machine program spectrum was developed using a five axis vertical machining center as the experimental object. Compared with existing programming methods, this method comprehensively considers multiple physical field coupling factors and is more suitable for the multi-stress collaborative loading requirements of precision preservation acceleration tests.