Abstract: In order to improve the controllability and real-time during the positioning process of aerospace monolithic reinforced frames, the positioning method of aerospace monolithic reinforced frames based on digital twin is proposed. The digital twin models of the positioned object and the flexible tooling are created, based on the process number that correspond to the assembly progress and the feedback on the state data of the end positioners on flexible tooling and the aerospace monolithic reinforced frames in practice. The magnitude of the contact forces and the coordinates of the monitoring points are analysed by “ANSYS” software, the positioning quality can be predicted, and the adjustment value can be optimized. In order to guarantee the adjusting movement for the design requirement and the operating security in practice, the adjusting movement of the end positioners on flexible tooling is monitored by “Unity3D” software. The controllability and real-time during the positioning process of aerospace monolithic reinforced frames can be improved.